Kati A. Chaffin

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Water samples from seven different sites at the Amnicola marsh in Chattanooga, TN, were analyzed for the amount of protein present. Four sets of samples were concentrated using three methods--one using an ultrafiltration cell, one using a lyophilizer, and two utilizing a household food dehydrator. Two protein assays, the Bradford assay and the Quantigold assay (two sets of samples each), were used to determine the amount of protein present by measuring the absorbance of light at 595 nm. These data were compared with data from the standard curves of bovine serum albumin (BSA) to estimate the amount of protein in the water samples. Results showed that very little protein, if any, was present in the water.

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