The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using AtPRP4F/4R, AtPRP4F/Lba1, and AtPRP4F/4R/Lba1 primer combinations. The PCR products were analyzed on 1% (w/v) agarose gels in TAE, visualized by ethidium bromide staining, and imaged with a UVP EC3 imaging system. We expected to identify homozygous plants for a T-DNA insertional mutation. This work is poised to help develop a tool that will grant us the ability to examine the function of AtPRP4 inside specific cell walls as well as uncover any potential phenotypes associated with the loss of this protein found in Arabidopsis thaliana.
Raitz, Sydney and Trott, Timothy D., "Isolation of Arabidopsis thaliana plants homozygous for an insertional inactivation mutation within atPRP4." (2022). Faculty Works. 12.